DYNAMICS OF PIGMENT ACCUMULATION IN THE LEAVES OF FABA BEANS (FABA BONA MEDIC.) UNDER THE INFLUENCE OF MICROBIAL PREPARATIONS

S. V. Pyda, I. I. Opatskyi, A. Yu. Dzendzel, O. V. Tryhuba

Abstract


This article examines the results of studies on the dynamics of accumulation of photosynthetic pigments in the leaves of Faba bona depending on the growth and development stages and pre-sowing seed treatment with microbial preparations. It was found that the contents of chlorophylls and carotenoids change significantly during plant ontogenesis. The highest total chlorophyll content was recorded at the green pod stage, while the lowest values were observed during the budding stage. The maximum carotenoid content in the leaf mesophyll was noted at the flowering stage, slightly lower at the green pod stage, and lowest during budding.
Pre-sowing inoculation of seeds with the microbial preparations Rhizobofit and Rhizohumin had a significant effect on the accumulation of photosynthetic pigments in the leaves throughout the entire growing period. Under the action of Rhizobofit at the budding stage, the chlorophyll a content increased statistically significantly by 12.2 % compared to the control, whereas the use of Rhizohumin ensured even higher values (by 17.9 %), which was due to the complex composition of the preparation. During flowering, both Rhizobofit and Rhizohumin statistically significantly increased the contents of chlorophylls a, b, and carotenoids.
At the green pod stage and at the beginning of pod maturation, both preparations significantly increased the contents of chlorophyll a and carotenoids; however, the maximum values were observed with the use of Rhizohumin. An increase in the proportion of chlorophyll a is an important indicator of enhanced plant resistance to stress factors, particularly under conditions of climate change.
Pre-sowing seed treatment with Rhizohumin significantly reduced the chlorophyll a to chlorophyll b ratio, indicating a more intensive effect of Rhizohumin on the accumulation of chlorophyll b in the leaf mesophyll of Faba bona compared to Rhizobofit.
Seed inoculation with microbial preparations contributed to improved nitrogen nutrition of plants as a result of activation of symbiotic fixation of molecular nitrogen, which directly affected the intensity of photosynthetic pigment biosynthesis. The obtained results indicate the expediency of using microbial preparations in the cultivation technology of Faba bona to optimize the photosynthetic activity of plants.

Keywords


Faba bona Medic.; microbial preparations; chlorophylls; carotenoids; pigment ratio

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DOI: https://doi.org/10.25128/2078-2357.25.4.9

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