THE INFLUENCE OF MICROBIAL PREPARATIONS AND FUNGICIDES ON THE PARAMETERS OF WATER EXCHANGE IN THE LEAVES OF FOOD LENTILS (LENS CULINARIS MEDIK.)

V. O. Kozak, S. V. Pyda

Abstract


This study investigated how treating Red lentil (Lens culinaris Medik.) seeds with the microbial preparation Rhizobophytes and various Rhizobium leguminosarum biovar viceae (R. leg) strains (C4-30, 724, F 11-2, F 16-1) affects water exchange in leaves during the bean formation phase. We also examined these effects when fungicides Laivit and Maxim were applied, both individually and in combination with the microbial treatments, for crops grown in the Western Forest-Steppe of Ukraine. Our research revealed that mono-treatment of seeds with microbial preparations, fungicides, or their combined use positively influenced several water exchange parameters: it impacted the water-holding capacity of cytoplasmic colloids; it reduced the water deficit in Lens culinaris leaves; it increased the total water content in leaf tissues.
Specifically, inoculating seeds with Rhizobophytes and R. leg strains in the presence of Laivit and Maxim fungicides significantly enhanced the water-holding capacity of leaf cytoplasmic colloids after 2 hours of wilting. The lowest water loss after 2 and 4 hours was observed in the variant where seeds were treated with the Maxim + R. leg C4-30 combination: 12.69 % ± 0.14 % after 2 hours; 23.23 % ± 0.40 % after 4 hours. This represents a 14.2 % and 22.0 % reduction, respectively, compared to the control group. For water loss after 24 hours, the lowest value was found with mono-inoculation of seeds with R. leg C4-30, showing a 5.9 % reduction compared to the control. Significant reductions in water loss after 24 hours of wilting were also observed when lentil seeds were inoculated with Rhizobophytes (2.4 %) and Rhizobium leguminosarum strains C4-30 (5.9 %), 724 (2.2 %), and F16-1 (1.9 %) compared to the control. Inoculating Lens culinaris seeds with microbial preparations prior to sowing, particularly when combined with fungicide treatment, led to a slight increase in the total water content in the leaves of the studied plants during the bean formation phase. The highest tissue watering was determined under the influence of the combined use of R. leg C4-30 with the fungicide Maxim. Furthermore, mono-treatment of seeds with the fungicide Laivit significantly reduced the water deficit of lentil leaves by 5.2 % compared to the control.

Keywords


food lentil (Lens culinaris Medik.); microbial preparations; fungicides; water regime; total water content; water retention capacity; water deficit

References


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DOI: https://doi.org/10.25128/2078-2357.25.1-2.7

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