STUDY OF THE ANATOMICAL STRUCTURE OF MISCANTHUS × GIGANTEUS J.M.GREEF & DEUTER EX HODKINSON & RENVOIZE LEAVES USING THE OPENFLEXURE MICROSCOPE

N. V. Herts, A, I. Herts, L. S. Barna

Abstract


This study demonstrates the potential of investigating and analyzing the anatomical structure of Miscanthus × giganteus (Giant Miscanthus) leaves using light microscopy with the OpenFlexure Microscope. Research on this crop is crucial for understanding its morphological and physiological characteristics, which influence productivity and adaptation to diverse growing conditions. Key features of leaf structure, including the epidermis, stomatal apparatus, and vascular system, determine the efficiency of photosynthesis, transpiration, and the accumulation of pollutants. These factors are essential for enhancing the productivity of this crop and optimizing its use in ecological projects, particularly in phytoremediation.
Accessible, functional, and high-precision tools are essential for the anatomical analysis of plants. The OpenFlexure Microscope facilitates high-resolution imaging and detailed examination of cellular structures in leaves, such as the epidermis, mesophyll, and veins, as well as their interactions. With its 3D-printed design and integration with digital imaging technologies, the OpenFlexure Microscope enhances access to microscopy, even in laboratories with limited resources or in field-based studies.
This article presents the anatomical features of Miscanthus × giganteus leaves, which are critical for further breeding and agronomic research. The advantages of using the OpenFlexure Microscope in botanical studies are highlighted, particularly in the examination of plants with complex morphological structures. The method allows for a comprehensive analysis of both microscopic and macroscopic leaf characteristics.
The findings indicate that the leaf structure of Miscanthus × giganteus is well-adapted to various climatic conditions, contributing to the plant’s resilience to stress factors. The study concludes that analyzing leaf anatomy is essential for advancing knowledge of the growth and development mechanisms of this crop. The results could contribute to the improvement of breeding programs and the development of effective cultivation strategies for Miscanthus in environmentally challenging sites.
Thus, the anatomical study of Miscanthus × giganteus using the OpenFlexure Microscope represents an important step toward optimizing its use as an energy crop and a phytoremediation tool. This approach combines economic benefits, ecological sustainability, and scientific innovation, addressing contemporary challenges in energy and environmental science. Integrating the OpenFlexure Microscope into research in Ukraine could significantly enhance local scientific capacity, improve the accessibility of modern tools for plant analysis, and provide higher-quality data for bioenergy and agricultural research.

Keywords


Miscanthus × giganteus; light microscopy; leaf anatomy; bulliform cells; Kranz anatomy; adaptation; OpenFlexure Microscope

References


Herts A. I., Kononchuk O. B., Herts N. V., Pidlisniuk V. V., Khomenchuk V. O., Pyda S. V. Aktyvnist fotosyntetychnoho aparatu Miscanthus×giganteus za umov zabrudnennia gruntu dyzelnym palnym i vnesennia biocharu. Fiziolohiia roslyn i henetyka. 2022. T. 54, No 2. S. 161–176. doi: https://doi.org/10.15407/frg2022.02.161. [in Ukrainian]

Humentyk M. Ya., Hayda Yu. I., Fuchylo Ya. D., Hnap I. V. Ekonomichna efektyvnist investytsii u vyroshchuvannia bioenerhetychnykh kultur v zoni Lisostepu Ukrainy. Ekonomichnyii analiz. 2018. T. 28, No 2. S. 21–29. [in Ukrainian]

Kovalyshyn I. B., Likhanov A. F., Pinchuk A. P., Vakhnovska N. H. Epiderma lystkiv dribnokvitkovykh predstavnykiv rodu Clematis L. Lisove i sadovo-parkove hospodarstvo. 2016. No 9. URL: http://nbuv.gov.ua/UJRN/licgoc_2016_9_6. [in Ukrainian]

Marchenko V. Enerhetychni kultury v Ukraini. Agroexpert. 2012. No 9. S. 114–117. [in Ukrainian]

Miskantus v Ukraini: monohrafiia / Roik M. V. ta in. Kyiv : FOP Yamchynskyi O. V., 2019. 256 s. [in Ukrainian]

Morhun I. A. Biolohichni osoblyvosti roslyn miskantusa za umov optymal'noho zvolozhennia. Novitni tekhnolohii vyroshchuvannia sil's'kohospodars'kykh kul'tur: tezy dopovidey 5 Mizhnarodnoi naukovo-praktychnoi konferentsii molodykh vchenykh (m. Kyiv, 29–30 veresnia 2016 r.). Vinnytsia, Nilan-LTD. 2016. S. 111. [in Ukrainian]

Briske D. D. Developmental Morphology and Physiology of Grasses. Grazing Management: An Ecological Perspective. Portland: Timber Press; 1991. pp. 85–108.

Barbosa B., Boléo S., Sidella S, Costa J., Duarte M. P., Mendes B., Salvatore L. Cosentino & A. L. Fernando Phytoremediation of Heavy Metal-Contaminated Soils Using the Perennial Energy Crops Miscanthus spp. and Arundo donax L. Bioenergy Res. 2015; Vol. 8, P.1500–1511.•doi:10.1007/s12155-015-9688-9.

Foster J. R, Smith W. K. Influence of stomatal distribution on transpiration in low‐wind environments. Plant Cell Environ. 1986; Vol. 9: P. 751–759. doi:10.1111/j.1365-3040.1986.tb02108.x.

Grant S. D., Richford K., Burdett H. L., McKee D., Patton B. R. Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation. R Soc Open Sci. 2020;7: 191921. doi:10.1098/rsos.191921.

Knapper J., Collins J. T., Stirling J., McDermott S., Wadsworth W., Bowman R. Fast, high precision autofocus on a motorised microscope: automating blood sample imaging on the OpenFlexure Microscope. arXiv [physics.ins-det]. 2021. doi:10.48550/ARXIV.2109.06842.

Lundgren M. R., Osborne C. P., Christin P-A. Deconstructing Kranz anatomy to understand C4 evolution. J Exp Bot. 2014;65: 3357–3369. doi:10.1093/jxb/eru186.

Nedukha O. Effects of moderate drought on leaf bulliform cells of aquatic and coastal population of Phragmites australis. Turk J Botany. 2022;46: 459–472. doi:10.55730/1300-008x.2722.

Nurzhanova A., Pidlisnyuk V., Abit K., Nurzhanov C., Kenessov B., Stefanovska T., et al. Comparative assessment of using Miscanthus × giganteus for remediation of soils contaminated by heavy metals: a case of military and mining sites. Environ Sci Pollut Res Int. 2019; Vol. 26: P. 13320–13333. doi:10.1007/s11356-019-04707-z.

Pauková Ž., Jureková Z. Stomatal density in Miscanthus leaves. Acta Hortic Regiotect. 2015; Vol. 18: Pp. 45–48. doi:10.1515/ahr-2015-0009.

Open Flexure microscope. URL: https://openflexure.org/projects/microscope/ (дата звернення: 26.01.2025).




DOI: https://doi.org/10.25128/2078-2357.24.3-4.1

Refbacks

  • There are currently no refbacks.


Creative Commons Attribution 4.0 License