EFFECT OF SEED TREATMENT WITH METABOLIC ACTIVE SUBSTANCES ON ASCORBATE PEROXIDASE ACTIVITY AND ASCORBATE CONTENT IN COMMON WHEAT (TRITICUM AESTIVUM L.) SPROUTS UNDER WATER DEFICIT CONDITIONS

Yu. M. Palivoda, V M. Havii

Abstract


The article presents a comparative analysis of the effects of metabolically active substances and their combinations on ascorbate peroxidase activity and ascorbate content in common wheat (Triticum aestivum L.) seedlings under water deficit conditions simulated using PEG 6000. Ten seed treatment options were studied: control (unprocessed seeds + distilled water); PEG 6000 solution (12 %); vitamin E solution (10-8M) – E; ubiquinone-10 solution (10-8M) – Q; methionine solution (0.001 %) – M; paraoxybenzoic acid (POBA) solution (0.001%) – P; MgSO4 solution (0.001%) – Mg; combinations: vitamin E (10-8M) + ubiquinone-10 (10-8M) – EQ; vitamin E (10-8M) + methionine (0.001 %) + POBA (0.001 %) – EMP; vitamin E (10-8M) + methionine (0.001 %) + POBA (0.001 %) + MgSO4 (0.001 %) – EMPMg. The experimental water deficit was simulated using a 12 % PEG 6000 solution.
It was found that simulated drought conditions induced a 63.1% increase in ascorbate peroxidase activity compared to the control. Pretreatment of wheat seeds with compound E resulted in a 65.5 % increase in ascorbate peroxidase activity compared to the control and a 2.4 % increase compared to seedlings under simulated water deficit conditions using PEG 6000.
The activity of ascorbate peroxidase in wheat seedlings after treatment with magnesium sulfate, ubiquinone-10, and the combination of vitamin E + ubiquinone-10 exceeded control values by 47.6 %, 35.7 %, and 31 %, respectively, but did not surpass the levels induced by PEG. This suggests a protective effect of these substances under water deficit conditions but does not completely mitigate the inhibitory effect of PEG 6000.
Under simulated water deficit conditions, the accumulation of ascorbate in seedling tissues exceeded control values by 29.1 %. Treatment with ubiquinone-10 increased ascorbate content by 46.3 %, while treatment with a combination of vitamin E + methionine + POBA + MgSO4 increased it by 24.2 %. Other treatments also showed increases: vitamin E + methionine + POBA (18.8 %), methionine (13 %), and vitamin E + ubiquinone-10 (7.4 %).
Pre-treatment of wheat seeds with metabolically active substances increased the activity of antioxidant enzymes in wheat seedlings, enhancing drought resistance in the Provintsialka variety. Therefore, seed treatment with these substances could serve as a component of grain crop cultivation technology under water deficit conditions.

Keywords


soft wheat; metabolically active substances; PEG 6000; vitamin E; ubiquinone-10; paraoxybenzoic acid; methionine; MgSO4; ascorbate peroxidase; ascorbate

References


Bilchuk V. S., Rossikhina-Halycha A. S. Askorbat-hlutationova zakhysna systema roslyn kukurudzy za dii ioniv nikeliu. Rehuliatorni mekhanizmy v biosystemakh. 2012. No 3 (2). S. 9–14. https://doi.org/10.15421/021225. [in Ukrainian]

Derzhavnyi reiestr sortiv roslyn, prydatnykh dlia poshyrennia v Ukraini na 2022 rik. [Chynnyi vid 2022-09-08]. Vyd. ofits. Kyiv, 2022. 332 s. [in Ukrainian]

Dziuba V., Kuchmenko O. Suchasni uiavlennia pro rol ubikhinonu v protsesakh metabolizmu klityny. Visnyk Lvivskoho universytetu. Seriia biolohichna. 2017. No 75. S. 3–13. [in Ukrainian]

Koziuchko A. H., Havii V. M., Kuchmenko O. B. Vplyv peredposivnoi obrobky nasinnia metabolichno aktyvnymy rechovynamy na okremi fiziolohichni pokaznyky soi sortu Annushka ta ii produktyvnist. Naukovi zapysky Ternopilskoho natsionalnoho pedahohichnoho universytetu imeni Volodymyra Hnatiuka. Ser. Biolohiia. 2020. Vyp. No 1–2 (79). C. 84–90. DOI: http://doi.org/10.25128/2078-2357.20.1-2.12. [in Ukrainian]

Kurylenko A. O., Kuchmenko O. B. Vplyv peredposivnoi obrobky nasinnia na vmist produktiv okyslennia lipidiv, vitaminiv ta aktyvnist antyoksydantnykh enzymiv v zerni ozymoho zhyta. Notatky suchasnoi biolohii. 2022. 1 (1). S. 18–22. DOI: 10.29038/2617-4723-2022-1-1-3. [in Ukrainian]

Palyvoda Yu. M., Havii V. M. Vplyv obrobky nasinnia metabolichno aktyvnymy rechovynamy na fotosyntetychnu produktyvnist prorostkiv pshenytsi miakoi (Triticum aestivum L.) za modeliuvannia vodnoho defitsytu. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Ahronomiia ta biolohiia. 2023. No 3 (49). S. 49–55. https://doi.org/10.32845/agrobio.2022.3.7. [in Ukrainian]

Sokolovska-Serhiienko O. H. Intensyvnist fotosyntezu ta aktyvnist antyoksydantnykh fermentiv lystkiv ozymoi pshenytsi za riznykh umov 107 mineralnoho zhyvlennia. Fiziolohiia i biokhimiia kulturnykh roslyn. 2013. T. 45, No 3. S. 206–212. [in Ukrainian]

Khomenko S. O. Posukhostiykist ta elementy produktyvnosti kolektsiynykh zrazkiv pshenytsi miakoi iaroi v umovakh Lisostepu Ukrainy. Myronivskyi visnyk. 2017. Vyp. 4. S. 79–87. [in Ukrainian]

Antonenko K., Duma M., Kreicberg V., Kunkulberga D. The influence of microelements selenium and cooper on the rye malt amylase activity and flour technological properties. Agronomy Research. 2016. Vol. 14 (2). Р. 1261–1270.

Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Ibid. 2006. Vol. 141(2). P. 391–396. https://doi.org/10.1104/pp.106.082040.

Ashraf M., Harris P. J. C. Photosynthesis under stressful environments: An overview. Photosynthetica. 2013. Vol. 51(2). Р. 163–190. https://doi.org/10.1007/s11099-013-0021-6.

Barkosky R. R., Einhellig F. A. Allelopathic interference of plant water relationships by para-hydroxybenzoic acid. Botanical Bulletin of Academia Sinica. 2003. Vol. 44. Р. 53–58.

Guo W., Chen S., Hussain N., Cong Y., Liang Z., Chen K. Magnesium stress signaling in plant: just a beginning. Plant Signal Behav. 2015. Vol. 3(10). e992287. https://doi.org/10.4161/15592324.2014.992287.

Hasanuzzaman M., Bhuyan M., Anee T. I., Parvin K., Nahar K., Mahmud J. A., Fujita M. Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants. 2019. Vol. 8(9). Р. 384. https://doi.org/10.3390/antiox8090384.

Kolesnikov M., Gerasko T., Paschenko Yu., Pokoptseva L., Onyschenko O., Kolesnikova A. Effect of water deficit on maize seeds (Zea mays L.) during germination. Agronomy Research, 2023. Vol. 21, № 1. Р. 156–174. DOI: http://doi.org/10.15159/AR.23.016.

Liu M, Lu S. Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering. Front Plant Sci. 2016. Vol. 7. P. 1898. https://doi.org/10.3389/fpls.2016.01898.

Martinez Y., Li X., Liu G. et al. The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids. 2017. 12(49). P. 2091–2098. https://doi.org/10.1007/s00726-017-2494-2.

Nakano Y., Asada K. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts. Plant Cell Physiol. 1981. Vol. 22. Р. 867–880.

Nezhadahmadi A., Prodhan Z. H., Faruq G. Drought Tolerance in Wheat. The Scientific World Journal. 2013. ID 610721. https://doi.org/10.1155/2013/610721.

Qasim Ali, Muhammad Tariq Javed, Muhammad Zulqurnain Haider, Noman Habib, Muhammad Rizwan, Rashida Perveen, Shafaqat Ali, Mohammed Nasser Alyemeni, Hamed A. El-Serehy and Fahad A. Al-Misned. α-Tocopherol Foliar Spray and Translocation Mediates Growth, Photosynthetic Pigments, Nutrient Uptake, and Oxidative Defense in Maize (Zea mays L.) under Drought Stress. Agronomy. 2020. Vol. 10(9), 1235. https://www.mdpi.com/2073-4395/10/9/1235.




DOI: https://doi.org/10.25128/2078-2357.24.1.10

Refbacks

  • There are currently no refbacks.


Creative Commons Attribution 4.0 License