O. I. Horyn, H. I. Falfushynska


Phytoextracts and natural antioxidants should provide promising results in therapeutic intervention for diabetes mellitus when they include into medical treatment scheme. The present study aimed to investigate the effect of Momordica charantia phytoextract alone and in complex-form with nano zinc oxide as well as zinc compounds on rate of hemolysis and oxidative stress parameters in Cyprinus carpio red blood cells (RBC) after their exposure to high glucose in vitro. The results have shown that the glucose treatment was capable to promote an increase in oxidative damage of lipids and proteins, the break of balance in antioxidant defence and enhance the rate of hemolysis and methylglyoxal concentration. When glucose-treated RBC were probed with M. charantia extract, nZnO- Momordica, nZnO and zinc picolinate specific response to different co-exposures was disclosed. Zinc picolinate in general had no significant effect on the studied parameters with few exceptions, then ZnO nanoparticles made glucose effects more profound. Momordica in herbal extract form and, particularly, in green synthetized ZnO nanoparticle have caused the decrease in lipid and protein peroxidation, glutathione trasferase activity, rate of hemolysis and methylglyoxal, and the increase in catalase and glutathione up to control baseline. These results have pointed to the necessity of further investigations of antihyperglycemic activity of Momordica and mechanistic explanation of its potentials.


Momordica charantia, green synthesis, ZnO nanoparticle, phytocomplex, oxidative stress, hemolysis


Aebi H. Catalase / H. Aebi // Methods Enzym Anal. — 1974. — Vol. 2. — P. 673—684.

Anderson M.E. Determination of glutathione and glutathione disulfide in biological samples / M.E. Anderson // Methods Enzymology. 1985. — Vol. 113. — P. 548 555.

Chaturvedi P. Momordica charantia maintains normal glucose levels and lipid profiles and prevents oxidative stress in diabetic rats subjected to chronic sucrose load / P. Chaturvedi, S. George // Journal of Medicinal Food. J Med Food. — 2010. — Vol. 13, issue 3. — P. 520—527.

Cruz K. J. C. Antioxidant role of zinc in diabetes mellitus / K. J. C. Cruz, A. R. S. de Oliveira, D.N. Marreiro // World Journal of Diabetes. — 2015. — Vol. 6, issue 2. — P. 333—337.

Evaluation of methylglyoxal toxicity in human erythrocytes, leukocytes and platelets, / A. S. Prestes, M.M. Dos Santos, A. Ecker [et al.] // Toxicology Mechanisms and Methods. — 2017. — Vol. 27, issue 4. — P. 307—317.

Giacco F. Oxidative stress and diabetic complications / F. Giacco, M. Brownlee // Circulation Research. — 2010. — Vol. 107, issue 9. — P. 1057—1070.

Gilbert R. P. Spectrophotometric determination of methylglyoxal with 2,4-dinitrophenylhydrazine / R. P. Gilbert, R. B. Brandt // Analytical Chemistry. — 1975. — Vol. 47, issue 14. — P. 2418—2422.

Glycation of human erythrocyte glutathione peroxidase: effect on the physical and kinetic properties / S. Suravajjala, M. Cohenford, L. R. Frost [et al.] // Clinica Chimica Acta. — 2013. — Vol. 421. — P. 170—176.

Habig W.H. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation / W.H. Habig, M.J. Pabst, W.B. Jakoby // Journal of Biological Chemistry. 1974. — Vol. 249. — P. 7130—7139.

James S. H. Microangiopathic hemolytic anemia as a complication of diabetes mellitus / S. H. James, A. M. Meyers // American Journal of the Medical Sciences. — 1998. — Vol. 315, issue 3. — P. 211—215.

Joseph B. Antidiabetic effects of Momordica charantia (bitter melon) and its medicinal potency / B. Joseph, D. Jini // Archive of "Asian Pacific Journal of Tropical Disease. — 2013. — Vol. 3, issue 2. — P. 93—102.

Lushchak V. I. Indices of oxidative stress. 1. TBA-reactive substances and carbonylproteins / V. I. Lushchak, T. V. Bahniukova, O. V. Lushchak // Ukrainskii Biokhimicheskii Zhurnal. — 2004. — Vol.73, issue 3, P. 136—141.

Methylglyoxal induces DNA crosslinks in ECV304 cells via a reactive oxygen species-independent protein carbonylation pathway / C. Y. Tu, Y. F. Chen, C. K. Lii [et al.] // Toxicology in Vitro. — 2013. — Vol. 27. — P. 1211—1219.

Momordica charantia and type 2 diabetes: from in vitro to human studies / S. D.Habicht, C. Ludwig, R.Y. Yang [et al.] // Current Diabetes Reviews. — 2014. — Vol. 10, issue 1. — P. 48—60.

Carassius auratus As a novel model for the hyperglycemia study/ H. Falfushynska, O. Horyn, L. Gnatyshyna [et al.] // Ukrainian Biochemical Journal. — 2019. — Vol. 91, issue 4. — P. 58—69.

Oxidative stress in diabetes mellitus: is there a role for hypoglycemic drugs and/or antioxidants / O.O. Erejuwa // Oxidative stress disorder. — 2012. — P. 217—246.

Persistent impaired glucose metabolism in a zebrafish hyperglycemia model / K.M. Capiotti, R.Jr. Antonioli, L.W. Kist [et al.] // Comparative Biochemistry and Physiology. — 2014. — Vol. 171B. — P. 58—65.

Ramesh R. Green Syntheis of Zinc Oxide Nanoparticles Using Flower Extract Cassia Auriculata / R. Ramesh, A. Rajendran, M. Meenakshisundaram // Journal of NanoScience and NanoTechnology. — 2014. — Vol. 2, issue 1. — P. 41—45.

Rehman K. Mechanism of Generation of Oxidative Stress and Pathophysiology of Type 2 Diabetes Mellitus: How Are They Interlinked? / K. Rehman, M.S.H.Akash // Journal Cell Biochemistry. — 2017. — Vol. 118 (11). — P. 3577—3585.

Rethinking glycolysis: on the biochemical logic of metabolic pathways / A. Bar-Even, A. Flamholz, E. Noor [et al.] // Nature Chemical Biology. — 2012. — Vol. 8. — P. 509—517.

Viskupicova J. Effect of high glucose concentrations on human erythrocytes in vitro / J. Viskupicova, D. Blaskovic, S. Galiniak [et al.] // Redox biology. — 2015. — Vol. 5. — P. 381—387.

Deklaratsiynyy patent Ukrainy na korysnu model' Sposib induktsii hiperhlikemii u koropovykh ryb / H.I. Fal'fushyns'ka, O. B. Stoliar, O. I. Horyn, V. V. Khoma, L. L. Hnatyshyna, H. B. Buiak — No u201811223; zaiavl. 15.11.2018. (In Ukrainian).



  • There are currently no refbacks.

Copyright (c) 2019 O. I. Horyn, H. I. Falfushynska

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.