THE CHANGES OF C-REACTIVE PROTEIN AND TUMOR NECROSIS FACTOR-α CONTENT IN RATS OF DIFFERENT AGE WITH MODELED SECOND-HAND TOBACCO SMOKING COMBINED WITH PROLONGED TREATMENT OF MONOSODIUM GLUTAMATE

N. V. Hetsko, I. Y. Krynytska

Abstract


The World Health Organization considers tobacco smoke as a global health and social problem. The active use of food supplements is ambiguous as regards human health and safety. So, the study of molecular mechanisms of toxicity of isolated and combined action of tobacco smoking and monosodium glutamate is one of the topical issues of modern science.
The study aimed to determine changes in the C-reactive protein and tumor necrosis factor-α of blood serum in rats under secondhand tobacco smoking combined with a long-term monosodium glutamate injection in the age aspect.
Experiments were carried out on 64 outbred white male rats divided into the following groups: I – control group; II – rats with modeled secondhand tobacco smoking; III - rats, which were injected with monosodium glutamate; IV - rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection. Blood serum TNF-α and CRP content were performed by enzyme-linked immunosorbent assay (ELISA) Kit developed by RayBiotech (USA) following the manufacturer’s protocol on a Multiscan FC analyzer (Finland).
The findings suggest that under the combined action of secondhand tobacco smoke and monosodium glutamate, the content of TNF-α increased by 2.9 times (p<0.001)) vs control group and by 32.3% (p<0.002) exceeded the data under the isolated action of secondhand tobacco smoke. Regarding the CRP, this indicator increased by 45.5% (p <0.001) vs control group. Secondhand tobacco smoke combined with monosodium glutamate use did not cause significant changes vs the isolated effect of tobacco smoke. As regards age, the systemic inflammatory response was more pronounced in immature rats.

Keywords


tobacco smoke; monosodium glutamate; age; inflammation

References


Al'-Akras R. K. M. Vliyaniye okislitel'nogo stressa na uroven' tsitokinov v kul'ture kletok. Mezhdunarodnyy nauchno-issledovatel'skiy zhurnal. 2014. Vyp. 4 (23). S. 78–80. [in Russian]

Abdrakhmanova A.I., Amirov N.B., Kim Z.F., Gayfullina R.F. S-reaktivnyy belok pri ostrom koronarnom sindrome: soderzhaniye v plazme i prognosticheskoye znacheniye. Vestnik sovremennoy klinicheskoy meditsiny. 2019. Vyp. 12(1). S.79–85. [in Russian]

Asmolov O. K., Rybak T. A., Smolʹsʹka I. M. Vplyv tyutyunopalinnya na patohenez khronichnykh obstruktyvnykh zakhvoryuvanʹ lehenʹ. Odesʹkyy medychnyy zhurnal. 2008. – Vyp. 6(110). S. 70–72. [in Ukrainian]

Asfandiyarova N. S. Nikotin i sistema immuniteta. Immunopatologiya, allergologiya, infektologiya. 2018. Vyp. 3. S. 6–12. [in Russian]

Bazdyrev Ye.D., Polikutina O.M., Kalichenko N.A., Slepynina YU.S., Uchasova Ye.G., Pavlova V.YU., Barbarash O.L. Vzaimosvyaz' kureniya s pokazatelyami sistemnogo vospaleniya u patsiyentov s ishemicheskoy bolezn'yu serdtsa. Klinicheskaya meditsina. 2017. Vyp.95(3). S.264–227. [in Russian]

Batrakova T.V., Vavilova T.V., Osipova N.A. Rol' ostrofaznykh belkov v diagnostike poslerodovogo endometrita (obzor literatury). Ginekologiya. 2016. – Vyp.18(1). S. 37–39. [in Russian]

Falaleyeva T. M., Samonina G. Ye., Beregovaya T. V. Dzyubenko N.V., Andreyeva L.A. Vliyaniye gliprolinov na strukturno-funktsional'noye sostoyaniye slizistoy obolochki zheludka i massu tela krys v usloviyakh dlitel'nogo vvedeniya glutamata natriya. Fizyka zhyvoho. 2010. – Vyp. 18 (1). S. 154–159. [in Russian]

Hlobalʹne opytuvannya doroslykh shchodo vzhyvannya tyutyunu (Global Adult Tobacco Survey – GATS). 2017. 240 s. [in Ukrainian]

Lizurchik L. V., Sheyda Ye. V. Vliyaniye tabachnogo dyma na soderzhaniye toksichnykh elementov v organizme krys. Vestnik OGU. 2014. Vyp. 6(167). S. 71–74. [in Russian]

Rudyk M. P., Pozur V. V., Opeyda YE. V., Voyeykova D. O., Khranovsʹka N. M., Fedorchuk O. H., Berehova T. V., Ostapchenko L. I. Modulyatorni efekty hlutamatu natriyu na funktsiyi tsyrkulyuyuchykh fahotsytarnykh klityn shchuriv in vivo ta in vitro. Dopov. Nats. akad. nauk Ukr. 2017. Vyp.5. S. 89–97. [in Ukrainian]

Samootsinka naselennyam stanu zdorovʺya ta rivnya dostupnosti okremykh vydiv medychnoyi dopomohy u 2018 rotsi. (za danymy vybirkovoho opytuvannya domohospodarstv u zhovtni 2018 roku). Elektronnyy resurs:http://www.ukrstat.gov.ua/druk/publicat/kat_u/2019/zb/03/zb_snsz2018pdf.pdf [in Ukrainian]

Shalʹmin O.S., Raznatovsʹka O. M. Rolʹ prozapalʹnykh tsytokiniv ta klityn leykotsytarnoyi formuly krovi u formuvanni imunnykh reaktsiy pry khimiorezystentnomu tuberkulʹozi lehenʹ. Suchasni medychni tekhnolohiyi. 2014. Vyp. 2. S. 83–89. [in Ukrainian]

Kucherenko D. Yu., Kucherenko I. S., Soldatkin O. O., Soldatkin A. P. Application of glutamate-sensitive biosensor for analysis of foodstuff. Biotechnologia Acta. 2018. Vol. 11(4). Р. 57–67.

Bhattacharya T., Ghosh S. K. Effect of neonatal exposure of monosodium glutamate in kidney of albino mice – a histological study. Nepal Med. Coll. J. 2019. Vol. 21(2). Р.134–141.

Barbieri S. S., Zacchi E., Amadio P., Gianellini S., Mussoni L., Weksler B. B., Tremoli E. Cytokines present in smokers’ serum interact with smoke components to enhance endothelial dysfunction. Cardiovascular Research. 2011. Vol. 90. P. 475–483.

Solomon U., Gabriel O. O., Henry E. O., Adrian I.O., Anthony T.E. Effect of monosodium glutamate on behavioural phenotypes, biomarkers of oxidative stress in brain tissues and liver enzymes in mice. World J. Neurosel. 2015. Vol. 5. Р. 339–349.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes: Council of Europe. Strasbourg. – 1986. Vol. 123. Р. 1–11.

Santiago H. A., Zamarioli A., Sousa M. D., Volpon J. B. Exposure to secondhand smoke impairs fracture healing in rats. Clin. Orthop. Relat. Res. 2017. Vol. 475(3). Р. 894–902.

Jha P., MacLennan M., Chaloupka F. J., Yurekli A., Ramasundarahettige C., Palipudi K., Zatońksi W., Asma S., Gupta P. C. Global hazards of tobacco and the benefits of smoking cessation and tobacco taxes. Disease Control Priorities. The World Bank. 2015. Vol. 3. P. 175–194.

Hou L., Huang J., Lu X., Wang L., Fan Z., Gu D. Polymorphisms of tumor necrosis factor alpha gene and coronary heart disease in a Chinese Han population: interaction with cigarette smoking. Thromb. Res. 2009. Vol. 123. Р. 822–826.

Shapiro H., Lutaty A., Ariel A. Macrophages, meta-inflammation, and immuno-metabolism. Scientific World J. 2011. Vol. 11. Р. 2509–2529.

Tawfik M. S., Al-Badr N. Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins C and E. Food and Nutrition Sciences. 2012. Vol. 3. Р. 651–659.

El-Demerdash F. M., Tousson E. M., Kurzepa J., Habib S. L. Xenobiotics, oxidative stress, and antioxidants. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018. Р. 1–2.

Yanbaeva D. G., Dentener M. A., Creutzberg E. C. Systemic effects of smoking. Chest. 2007. Vol. 131 (5). Р. 1557–1566.

Zhang Y., Zhang L., Venkitasamy C. Potential effects of umami ingredients on human health: Pros and cons. Critical Reviews in Food Science and Nutrition. 2019. Vol. 4. Р. 1–9.




DOI: https://doi.org/10.25128/2078-2357.20.1-2.7

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 N. V. Hetsko, I. Y. Krynytska

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.