THE CHANGES OF C-REACTIVE PROTEIN AND TUMOR NECROSIS FACTOR-α CONTENT IN RATS OF DIFFERENT AGE WITH MODELED SECOND-HAND TOBACCO SMOKING COMBINED WITH PROLONGED TREATMENT OF MONOSODIUM GLUTAMATE
Abstract
The study aimed to determine changes in the C-reactive protein and tumor necrosis factor-α of blood serum in rats under secondhand tobacco smoking combined with a long-term monosodium glutamate injection in the age aspect.
Experiments were carried out on 64 outbred white male rats divided into the following groups: I – control group; II – rats with modeled secondhand tobacco smoking; III - rats, which were injected with monosodium glutamate; IV - rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection. Blood serum TNF-α and CRP content were performed by enzyme-linked immunosorbent assay (ELISA) Kit developed by RayBiotech (USA) following the manufacturer’s protocol on a Multiscan FC analyzer (Finland).
The findings suggest that under the combined action of secondhand tobacco smoke and monosodium glutamate, the content of TNF-α increased by 2.9 times (p<0.001)) vs control group and by 32.3% (p<0.002) exceeded the data under the isolated action of secondhand tobacco smoke. Regarding the CRP, this indicator increased by 45.5% (p <0.001) vs control group. Secondhand tobacco smoke combined with monosodium glutamate use did not cause significant changes vs the isolated effect of tobacco smoke. As regards age, the systemic inflammatory response was more pronounced in immature rats.
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DOI: https://doi.org/10.25128/2078-2357.20.1-2.7
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