RHIZOGENESIS OF SILENE HYPANICA KLOKOV EXPLANTS AND THEIR ADAPTATION TO EX VITRO CONDITIONS

L. L. Dzhus, L. A. Koldar

Abstract


Among the conservation measures related to the preservation of plant diversity, an important place belongs to the creation and preservation of rare and endangered species in the crop conditions. Thus, the species Silene hypanica Klokov is under threat of extinction. This relict and endemic species is listed in the Red book of Ukraine, the European Red list, and 6th Resolution of the Berne Convention. Prospects for the conservation of this species are related to the study of its viability and require the development of effective methods of reproduction, in particular in vitro crop. The article presents the results of studies of one of the breeding stages in vitro – rhizogenesis of S. hypanica esplants and its dependence on the concentrations of β-indolyl butyric acid in nutrient media (0,1; 0,5; 1,0; 1,5; 2,0 mg / l), followed by adaptation of regenerating plants to ex vitro conditions. The study showed that the active process of rhizogenesis occurred due to the addition of β-IMC to the nutrient medium at a concentration of 0.5 mg/l and contributed to the production of 86.4 % of rooted regenerating plants.
Transplanting rooted plants into peat disks and then growing them in containers contributed to the successful adaptation of rooted plants.

Keywords


Silene hypanica; microclonal breeding; β-indolyl butyric acid; concentrations; regenerating plants; rooting; adaptation; substrate

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DOI: https://doi.org/10.25128/2078-2357.20.1-2.6

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