THE APPLICATION OF THE NOVEL INTEGRATIVE INDEX OF OXIDATIVE STRESS IN THE ASSESSMENT OF ENVIRONMENTAL IMPACT ON FRESHWATER MOLLUSKS

O. B. Stoliar, L. L. Gnatyshyna, V. V. Khoma, G. H. Springe

Abstract


The adverse environmental impacts cause the oxidative stress responsein the aquatic animals. However, depending on the severity and duration of impact, this response can be highly different. The aim of this study was the analysis of available results of the evaluation of antioxidant activities in the freshwater mollusks in the sense of the successfulness of the oxidative stress response. The recently proposed integrative index ‘Preparation to the oxidative stress’ (POS) was applied. Three populations of bivalve mollusks from the basin of the river Dniester were compared during three seasons, and in their ability to withstand heating (25o C and 30o C during 14 days) and exposure to ionizing radiation (14 days after the acute exposure to 2 mGy). The musselswere sampled in the low disturbed pristine site, highly polluted agricultural region and the cooling pond of the nuclear power plant with the constantly elevated temperature. The parameters for the calculation of POS included superoxide dismutase activity, catalase activity, glutathione S-transferase activity, glutathione concentration, and metallothionein (from its thiol groups) concentration. The values were calculated as the magnitude of change (as % change) in comparison to the corresponding control (less disturbed field group or non-exposed group). Three criteria for POS were applied. The number of the positive and negative changes and their limits were indicated. The analysis have shown that the POS responses were in the limits of adaptive ability in all studied cases. However, the results of POS calculation allowed the distinguishing of the responses that arerealized in the field and experimental exposures of mollusks.The most distinct responses were shown for the glutathione (mainly positive changes) whereas the metalothionein level was mainly oppressed, particularly under the heating. The depressive direction was estimated in the cases of extreme temperatures, irradiation and, mainly for the mollusks from the highly polluted sites. The key importance of POS as a survival strategy of the mussels exposed to adverse impact depending on the life historyis evident.


Keywords


Antioxidants; Reactive oxygen species; Oxidative stress; Integrative indexes; Bivalve mollusks

References


Ács A., Vehovszky Á., Győri J., Farkas A. Seasonal and size-related variation of subcellular biomarkers in quagga mussels (Dreissena bugensis) inhabiting sites affected by moderate contamination with complex mixtures of pollutants. Environ. Monit. Assess. 2016. Vol. 188, №7. P. 426. DOI: https://doi.org/10.1007/s10661-016-5432-y

Bhagat J., Ingole B. S., Singh N. Glutathione S-transferase, catalase, superoxide dismutase, glutathione peroxidase, and lipid peroxidation as biomarkers of oxidative stress in snails: A review. Invertebrate Survival Journal. 2016.Vol. 13. P. 336–349.

Biagianti-Risbourg S., Paris-Palacios S., Mouneyrac C., Amiard-Triquet C. Pollution Acclimation, Adaptation, Resistance, and Tolerance in Ecotoxicology. Encyclopedia of Aquatic Ecotoxicology. Dordrecht : Springer, 2013. P. 883–892. DOI: https://doi.org/10.1007/978-94-007-5704-2_81

Bolotov I. N., Makhrov A. A., Gofarov M. Y., Aksenova O. V., Aspholm P. E., Bespalaya Y., Kabakov M. V., Kolosova Y. S., Kondakov A. V., Ofenböck T., Ostrovsky A. N., Popov I. Yu., von Proschwitz T., Rudzite M., Rudzitis M., Sokolova S. E., Valovirta I., Vikhrev I. V., Vinarsky M. V., Zotin A. A. Climate warming as a possible trigger of keystone mussel population decline in oligotrophic rivers at the continental scale. Scientific Reports. 2018. Vol. 8, № 35. P. 1–9. DOI:https://doi.org/10.1038/s41598-017-18873-y

Canesi L., Corsi I. Effects of nanomaterials on marine invertebrates. Sci. Total. Environ. 2016. Vol. 565. P. 933−940. DOI: https://doi.org/10.1016/j.scitotenv.2016.01.085

Doyle J. J.,Ward J. E., Mason R. Exposure of bivalve shellfish to titania nanoparticles under an environmental-spill scenario: Encounter, ingestion and egestion. Journal of the Marine Biological Association of the UK. 2016. Vol. 96, №1. Р. 137−149. DOI: https://doi.org/10.1017/S0025315415001174

Falfushynska H. I., Gnatyshyna L. L., Golubev A. P., Stoliar O. B. Main partitioning criteria for the characterization of the health status in the freshwater mussels Anodonta cygnea from spontaneously polluted area in Western Ukraine. Environmental Toxicology.2012. Vol.27, № 8.P. 485–494.

Falfushynska H., Gnatyshyna L., Yurchak I., Ivanina A., Stoliar O., Sokolova I. Habitat pollution and thermal regime modify molecular stress responses to elevated temperature in freshwater mussels (Anodonta anatina: Unionidae). Science of the Total Environment. 2014. Vol. 500–501. P. 339–350.

Falfushynska H., Gnatyshyna L., Yurchak I., Stoliar O., Sokolova I. M. Interpopulational variability of molecular responses to ionizing radiation in freshwater bivalves Anodonta anatina (Unionidae). ScienceoftheTotalEnvironment.2016. Vol. 568. P. 444–456.

Gnatyshyna L., Falfushynska H., Bodilovska O., Oleynik O., Golubev A., Stoliar O. Metallothionein and glutathionein Lymnaea stagnalis determine the specifi city of responses on the effects of ionis in gradiation. Radioprotection. 2012 .Vol. 47, № 2. P. 231–242.

Lydeard C., Cowie R. H., Ponder W. F., Bogan A. E., Bouchet P., Clark S. A., Cummings K. S., Frest T. J., Gargominy O., Herbert D.G., Hershler R., Perez K. E., Roth B., Seddon M., Strong E. E., Thompson F. G. The global decline of nonmarine mollusks. Bio. Science. 2004. Vol. 54, № 4. P. 321–330. DOI: https://doi.org/10.1641/0006-3568(2004)054[0321:TGDONM]2.0.CO;2

Mischuk Y.V., Stoliar O. B. Peculiarities of metallothioneins of the bivalve mollusc Anodonta cygnea L. in the natural and laboratory living conditions. Hydrobiol. J. 2009. Vol. 45. P. 63–71. DOI: https://doi.org/10.1615/HydrobJ.v45.i5.70.

Moreira D. C., Venancio L. P. R., Sabino M. A. C. T., Hermes-Lima M. How widespread is preparation for oxidative stress in the animal kingdom? Comp. Biochem. Phys. 2016. Vol. 200A. P. 64–78.

Newton T. J., Cope W. G. Biomarker responses of unionid mussels to environmental contaminants. Freshwater Bivalve Ecotoxicology. Boca Raton : CRC Press, 2007. P. 257–284.

Paital B., Panda S. K., Hati A. K., Mohanty B., Mohapatra M. K., Kanungo S., Chainy G. B. N. Longevity of animals under reactive oxygen species stress and disease susceptibility due to global warming.World. J. Biol. Chem. 2016. Vol. 7, № 1. P. 110–127. DOI: https://doi.org/10.4331/wjbc.v7.i1.110

Ruttkay-Nedecky B., Nejdl L., Gumulec J., Zitka O., Masarik M., Eckschlager T., Stiborova M., Adam V., Kizek R. The role of metallothionein in oxidative stress. Int. J. Mol. Sci. 2013. Vol. 14. P. 6044‒6066. DOI: https://doi.org/ 10.3390/ijms14036044.

Springe G., Grinbega L.,Briede A. The role of the hydrological and hydromorphological factors in the forming of ecological quality of the medium-sized low land streams. Hydrology Research. 2009. Vol. 41, № 3–4. P. 330–337.

Stoliar O. B., Lushchak V. I. Environmental Pollution and Oxidative Stress in Fish. Oxidative Stress - Environmental Induction and Dietary Antioxidants. 2012. P. 131–166. URL: http://www.intechopen.com/books/oxidative-stress-environmental-induction-and-dietary-antioxidants/environmental-pollution-and-oxidative-stress-in-fish

Zhang J., Wang X., VikashV., Ye Q., Wu D., Liu Y., Dong W. ROS and ROS-Mediated Cellular Signaling. Oxid. Med. Cell. Longev. 2016. Vol. 2016. DOI:http://dx.doi.org/10.1155/2016/4350965

Masikevych Yu. H., Shestopalov O. V., Nehadaylo ta in.Teoriia system v ekolohii :pidruchnyk. Sumy :Sums'kyy derzhavnyy universytet, 2015. 330 s. (In Ukrainian).




DOI: https://doi.org/10.25128/2078-2357.19.1.6

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 O. B. Stoliar, L. L. Gnatyshyna, V. V. Khoma, G. H. Springe

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.